控制水稻中胚轴伸长的QTL定位

doi:10.3969/j.issn.1006-8082.2019.06.014

摘要/Abstract

摘要：

水稻中胚轴能够为幼苗破土提供动力，培育长中胚轴水稻品种有助于水稻直播技术的推广，因此，研究水稻中胚轴伸长具有重要的理论和现实意义。为分析调控水稻中胚轴伸长的遗传基础，以Asominori和IR24重组自交系（RIL）群体为材料，结合其连锁图谱，对2017年杭州收获种子的中胚轴长度性状进行QTL定位。结果表明，在Asominori/IR24重组自交系群体中共检测到3个控制中胚轴伸长的QTL位点，分别位于第2、第3和第7条染色体上，LOD值在2.34~3.41之间，单个QTL对表型贡献率在7.25%~11.07%之间。同时用Asominori遗传背景的IR24染色体片段代换系进行验证，qML2对应的代换家系CSSL12的中胚轴与Asominori相比显著伸长，qML7对应的代换家系CSSL37的中胚轴与Asominori相比显著缩短，从而验证了qML2和qML7位点的存在。与先前研究比较，qML3和qML7在不同群体不同环境下稳定表达。同时利用重组自交系群体对2018年杭州大田环境株高性状进行QTL定位，共检测到2个QTL位点，均与中胚轴QTL位点不重合，说明控制中胚轴伸长与控制株高有着不同的遗传基础。

关键词: 水稻, 中胚轴, QTL, 破土能力, 直播

Abstract:

The Rice mesocotyl can provide the power for seedling soilbreaking. The rice varieties with long mesocotyl could promote the popularization of rice direct seeding technology. Therefore, it is of great theoretical and practical significance to study rice mesocotyl elongation. To analyze the genetic basis of rice mesocotyl elongation, the Asominori and IR24 recombinant inbred lines (RIL) and their linkage maps were used for the experiment. The seeds gained in 2017 in Hangzhou were cultivated and measured the length of the mesocotyl for the QTLs mapping for mesocotyl length. Three QTLs were detected which were located on chromosomes 2, 3 and 7 respectively, and the LOD ranged from 2.34 to 3.41. The contribution rate of QTL to phenotype is between 7.25% and 11.07%. At the same time, the result was tested by a series of single segment substitution lines. The CSSL12 corresponding to qML2 was significantly elongated compared with Asominori, and the CSSL37 mesocotyl corresponding to qML7 significantly shortened, which proved the result of the QTL qML2 and qML7 to be right. At the same time, QTL mapping of plant height trait was carried out and two QTLs were detected, which were not coincident with QTLs for the length of mesocotyl, which indicating that controlling mesocotyl elongation and controlling plant height have different genetic basis.

Key words: rice, mesocotyl, QTL, soil breaking ability, direct seeding

中图分类号:

S511

牛世朋,吕育松,邬亚文,魏祥进,圣忠华,焦桂爱,胡时开,唐绍清*. 控制水稻中胚轴伸长的QTL定位[J]. 中国稻米, DOI: 10.3969/j.issn.1006-8082.2019.06.014 .

参考文献

[1]    黄成. 水稻中胚轴长度QTL分析[J]. 作物学报，2010，36（7）：
1 108-1 113.
[2]    姜洁锋，金林灿，王利润. 水稻中胚轴伸长研究进展[J]. 江西农业学报，2017，29（12）：27-30.
[3]    章秀福，朱德峰. 中国直播稻生产现状与前景展望[J]. 中国稻米，1996，2（5）：1-4.
[4]    林建荣，张光恒，吴明国，等. 水稻中胚轴伸长特性的遗传分析[J]. 作物学报，2006，32（2）：249-252.
[5]    马殿荣，孔德秀，陈温福，等. 杂草稻中胚轴伸长动态及其与籽粒淀粉酶活性和可溶性糖含量的关系[J]. 中国水稻科学，2014，28（1）：97-112.
[6]    曹立勇，朱军，延启传，等. 水稻籼粳交DH群体幼苗中胚轴长度的QTLs定位和上位性分析[J]. 中国水稻科学，2002，16（3）：221-224.
[7]    LU Q, ZHANG M, NIU X, et al． Uncovering novel loci for mesocotyl elongation and shoot length in indica rice through genome-wide association mapping[J]. Planta, 2016, 243（3）: 645-657．
[8]    WU J H, FENG F J, LIAN X M, et al. Genome-wide Association Study （GWAS） of mesocotyl elongation based on re-sequencing approach in rice[J]. BMC Plant Biol, 2015, 15（1）: 218.
[9]    马殿荣，孔德秀，刘晓亮. 杂草稻中胚轴伸长动态及其与籽粒淀粉酶活性和可溶性糖含量的关系[J]. 中国水稻科学，2014，28（1）：97-102.
[10] 杜金友，张桂荣，蔡爱军，等. 玉米中胚轴长度与内源激素关系的研究[J]. 玉米科学，2008， 16（3）：70-73.
[11] 王莹，马殿荣，陈温福. 北方杂草稻中胚轴伸长特性的初步研究[J]. 中国稻米，2008，14（3）：47-49.
[12] XIONG Q, MA B, et al. Ethylene-inhibited jasmonic acid biosynthesis promotes Mesocotyl/Coleoptile elongation of etiolated rice seedlings[J]. Plant Cell, 2017, 29（5）: 1 053-1 072.
[13] LUO X M, LIN W H, et al. Integration of light and brassinosteroid signaling pathways by a GATA transcription factor in Arabidopsis[J]. Developmental Cell, 2010, 19（6）: 872-883.
[14] TSUNEMATU, HIROSHI, YOSHIMURA, et al. RFLP framework map using recombinant inbred lines in rice [J]. Jpn J Breed, 1996, 194（2）: 279-284.
[15] KUBO T, AIDA Y, et al. Reciprocal chromosome segment substitution series devived from Japonica and Indica cross of rice [J]. Breed Sci, 2002, 52（12）: 319-325.
[16] 陈冰孀，李继洪，李淑杰，等. 不同类型高粱中胚轴伸长特性的研究[J]. 作物杂志，2016（3）：63-67.
[17] 王竹林，刘曙东，奚亚军. WinQTLCart2.0使用程序和QTL分析新方法—关联分析[J].安徽农业科学，2014，42（31）：10 858 -    10 860.
[18] MCCOUCH S, CHO Y, YANO M, et al. Report on QTL nomenclature [J]. Rice Genet Newsl, 1997, 14: 11-13.
[19] REDOnA E D, MACKILL D J. Mapping quantitative trait loci for seeding vigor in rice using RFLPs [J]. Theor Appl Genet, 1996, 92（3-4）: 395-402.
[20] DILDAY R H. Contribution of ancestral lines in the development of new cultivars of rice[J]. Crop Sci, 1990, 30（4）: 905-911.
[21] 曹立勇，袁守江，周海鹏，等. 外源激素对水稻中胚轴伸长的影响[J]. 作物学报，2005，31（8）：1 098-1 100.